The Application of Sintered (Fritted) Glass Filters to Bacteriological Work.
نویسندگان
چکیده
Glass disks, prepared by sintering finely pulverized glass in a suitable mold (Bruce and Bent, 1931; Kirk, Craig and Rosenfels, 1934; Shatenshtein, 1929) have found applications in the fields of chemistry (Prausnitz, 1924, 1929 and 1932), microchemistry (Kirk, 1933) and pharmacy (Prausnitz, 1930). As far as is known, there is no account in the literature of their employment in bacteriological work, except for the statement (Kirk, 1937) that they have been used satisfactorily for the sterilization of Tyrode's solution, peptic digests of protein and peptone and other materials of a similar nature. Very often in bacteriological work it is necessary to filter a small amount of material or to have a filter which is chemically clean as well as sterile. In immunological work it is imperative that filters be free from all foreign antigens. Sintered glass filters present these advantages, if they can be depended upon to withhold bacteria. The filters1 used in our work are of Jena glass of the type shown in figure 1. The sintered glass disk, 30 mm. in diameter, is of "5 auf 3" porosity. The capacity of the funnel above the sintered glass disk is 30 cc. The funnel is fitted into a test tube with a side arm by means of a rubber stopper. After plugging the side arm with cotton, the assembly is autoclaved at 120'C. for 30 minutes. A pressure of about 75 cm. of mercury is applied during the filtration process. After use, the funnels are disconnected from the test tube, placed in distilled water and boiled
منابع مشابه
THE COMPARISON OF MECHANICAL PROPERTIES OF SINTERED ?. QUARTZ SOLID SOLUTION AND GAHNITE GLASS – CERAMICS IN ZNO-AL2O3-SIO2 SYSTEM
The effect of precipitation of ?.qss. and gahnite phases during heat treatment of glass frits in the ZnO-Al2O3-SiO2 system on the mechanical property of resulting glass-ceramic specimens were investigated. It was shown that gahnite glass-ceramics had higher bend strength and toughness values than ?.qss. ones. The results are attributed to the higher modulus of elasticity as well as higher therm...
متن کاملAn investigation on Mechanical Properties of Apatite-Wollastonite-Diopside Glass-Ceramics Composites
Apatite-wollastonite (A-W)-phlogopite glass-ceramic is considered to be difficult to resorb, but often, it has been incorporated in particulate form to create new bioactive composites for potential maxillofacial applications. With various compositions, the present work has attempted to prepare apatite-wollastonite (A-W)-phlogopite glass ceramic composites, by applying sintering. Here, three-poi...
متن کاملFabrication, Characterization and Osteoblast Response of Cobalt-Based Alloy/Nano Bioactive Glass Composites
IIn this work, cobalt-based alloy/ nano bioactive glass (NBG) composites with 10, 15 and 20 wt% NBG were prepared and their bioactivity after immersion in simulated body fluid (SBF) for 1 to 4 weeks was studied. The scanning electron microscopy images of two- step sintered composites revealed a relatively dense microstructure the density of which decreased with the increase in the NBG amount. M...
متن کاملCHARACTERIZATION OF SHORT E-GLASS FIBER REINFORCEDGRAPHITE AND BRONZE FILLED EPOXY MATRIX COMPOSITES
The mechanical characterization of short E- glass fiber reinforced, graphite and sintered bronze filled epoxy composite was carried out in this study. The aim of the present study was to develop tribological engineering material. In this study the flexural strength, theoretical and experimental density, Hardness and Impact strength of composites was investigated experimentally. The results show...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Journal of bacteriology
دوره 34 5 شماره
صفحات -
تاریخ انتشار 1937